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KMID : 0191119970120060505
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Journal of Korean Medical Science
1997 Volume.12 No. 6 p.505 ~ p.513
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Molecular genetic characterization of alternatively spliced CD44 transcripts in human stomach carcinoma
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Kim YS
Chi SG/Kim YW/Park YK/Yoon C
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Abstract
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CD44 is a member of cell surface glycoproteins which are involved in cell-matrix adhesion and tumor
metastasis. Certain types of tumors express complex CD44 isoforms generated by alternative splicing of
2v-10v exons, and their expression appears to promote metastasis of tumor cells. Using a nested RT-PCR,
we analyzed expression of CD44 variants in 26 stomach carcinoma, 21 matched normal tissues, and 2 carcinoma
cell lines. We observed frequent and complex patterns of CD44 variant expression in tumor tissues. While
exons 6v and 7v expression was detected in most normal and tumor tissues, exon 9v was most rarely detected.
Exon 5v showed a significantly frequent expression in carcinoma, suggesting that its expression might contribute
to the malignant progression. While exon 9v was frequently observed in diffuse-type tumors, the other 8 variant
exons including 6v showed more frequent expression in intestinal-type tumors. Exons 9v and 10v were predominantly
expressed in advanced tumor tissues and exon 8v was expressed more frequently in tumors of lymph node metastasis.
We believe that series with a longer follow-up now need to be tested to clarify the association between CD44
splice variant expression and distant metastasis or long-term prognosis.
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